Pooled optical screening in bacteria using chromosomally expressed barcodes

Soares, R. R. G.; Garcia-Soriano, D. A.; Larsson, J.; Fange, D.; Schirman, D.; Grillo, M.; Knöppel, A.; Sen, B. C.; Svahn, F.; Zikrin, S.; Nilsson, M.; Elf, J.

doi:10.1101/2023.11.17.567382

bioRxiv · 10.1101/2023.11.17.567382

Pooled optical screening in bacteria using chromosomally expressed barcodes

Soares, R. R. G.·Garcia-Soriano, D. A.·Larsson, J.·Fange, D.·Schirman, D.·Grillo, M.·Knöppel, A.·Sen, B. C.·Svahn, F.·Zikrin, S.·Nilsson, M.·Elf, J.

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Abstract

Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not been possible in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia. coli. The method is applied to measure the intracellular maturation time of 81 red fluorescent proteins.

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Soares, R. R. G., Garcia-Soriano, D. A., Larsson, J., Fange, D., Schirman, D., Grillo, M., Knöppel, A., Sen, B. C., Svahn, F., Zikrin, S., Nilsson, M., Elf, J.. 2023-11-17. Pooled optical screening in bacteria using chromosomally expressed barcodes. https://doi.org/10.1101/2023.11.17.567382

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