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bioRxiv · 10.1101/2023.11.13.566747

High quality, high throughput, and low-cost simultaneous video recording of 60 animals in operant chambers using PiRATeMC

Ramborger, J.·Kalra, S.·Smith, A. C.·George, O.
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Abstract

BackgroundThe development of Raspberry Pi-based recording devices for video analyses of drug self-administration studies has shown to be promising in terms of affordability, customizability, and capacity to extract in-depth behavioral patterns. Yet, most video recording systems are limited to a few cameras making them incompatible with large-scale studies. New MethodWe expanded the PiRATeMC (Pi-based Remote Acquisition Technology for Motion Capture) recording system by increasing its scale, modifying its code, and adding equipment to accommodate large-scale video acquisition, accompanied by data on the throughput capabilities, video fidelity, synchronicity of devices, and comparisons between the Raspberry Pi 3B+ and 4B models. ResultsUsing PiRATeMC default recording parameters resulted in minimal storage ([~]350MB/h), high throughput (< [~]120 seconds/Pi), high video fidelity, and synchronicity within [~]0.02 seconds, affording the ability to simultaneously record 60 animals in individual self-administration chambers at a fraction of current commercial costs. No consequential differences were found between Raspberry Pi 3B+ and 4B models. Comparison with Existing Method(s)This system allows greater acquisition of video data simultaneously than other video recording systems by an order of magnitude with less storage needs and lower costs. Additionally, we report in-depth quantitative assessments of throughput, fidelity, and synchronicity, displaying real-time system capabilities. ConclusionsThe system presented is able to be fully installed in a months time by a single technician and provides a scalable, low cost, and quality-assured procedure with a high-degree of customization and synchronicity between recording devices, capable of recording a large number of subjects with high turnover in a variety of species and settings.

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Ramborger, J., Kalra, S., Smith, A. C., George, O.. 2023-11-16. High quality, high throughput, and low-cost simultaneous video recording of 60 animals in operant chambers using PiRATeMC. https://doi.org/10.1101/2023.11.13.566747

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