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bioRxiv · 10.1101/2023.11.08.566240

Divergent Protein Kinase A contributes to the regulation of flagellar waveform of the motile flagellum of Leishmania mexicana

Fochler, S.·Walker, B. J.·Wheeler, R. J.·Gluenz, E.
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Abstract

The second messenger cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) have been implicated in the regulation of flagellar motility in diverse eukaryotes, yet the specific contributions of PKA isoforms remain unclear. Moreover, the kinetoplastid PKA is insensitive to cAMP. Here we investigated subcellular localisations of PKA subunits in Leishmania and study the flagellar waveform parameters in PKA deletion mutants. Expansion microscopy localised LmxPKAR1 between the paraflagellar rod and the axoneme and LmxPKAC1, LmxPKAC2 depend on LmxPKAR1 for enrichment in the flagellum. LmxPKAC3 requires LmxPKAR3 for co-localisation at the cell cortex. Deletion of LmxPKAR3 resulted in increased flagellar LmxPKAC3 signal and increased swimming speed. Conversely, deletion of LmxPKAC3 resulted in reduced flagellar beat frequencies and swimming speed. The absence of LmxPKAC1 resulted in a lower incidence of high frequency tip-to-base waveforms, and consequently reduced swimming speed. LmxPKAC1 dissociated from cytoskeletons upon addition of guanosine, adenosine or inosine, consistent with a novel nucleoside-based PKA activation mechanism. These data suggest that this divergent PKA pathway regulates the motility of Leishmania through the modulation of flagellar waveforms.

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Fochler, S., Walker, B. J., Wheeler, R. J., Gluenz, E.. 2023-11-09. Divergent Protein Kinase A contributes to the regulation of flagellar waveform of the motile flagellum of Leishmania mexicana. https://doi.org/10.1101/2023.11.08.566240

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