Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein

Chan, S. H.; Tirole, L.; Kneller, D. W.; Kelley, T. M.; Dai, N.; Robb, G. B.

doi:10.1101/2023.10.28.564488

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bioRxiv · 10.1101/2023.10.28.564488

Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein

Chan, S. H.·Tirole, L.·Kneller, D. W.·Kelley, T. M.·Dai, N.·Robb, G. B.

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Abstract

mRNA vaccines and therapeutics are highly effective and can be developed and manufactured with a relatively short lead time. Here we report an all-enzyme platform to generate capped synthetic RNA in a one-step process based on an RNA capping enzyme-T7 RNA polymerase fusion protein. Under standard in vitro transcription reaction conditions, the fusion protein, in conjunction with an RNA cap 2'-O-methyltransferase, can generate synthetic mRNA with up to 95% of Cap-1 incorporation, greatly simplifying mRNA manufacturing workflows.

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Chan, S. H., Tirole, L., Kneller, D. W., Kelley, T. M., Dai, N., Robb, G. B.. 2023-10-28. Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein. https://doi.org/10.1101/2023.10.28.564488

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