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bioRxiv · 10.1101/2023.10.25.564042

A reinterpretation of the relationship between persistent and resurgent sodium currents

Brown, S. P.·Lawson, R. J.·Moreno, J. D.·Ransdell, J. L.
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Abstract

The resurgent sodium current (INaR) activates on membrane repolarization, such as during the downstroke of neuronal action potentials. Due to its unique activation properties, INaR is thought to drive high rates of repetitive neuronal firing. However, INaR is often studied in combination with the persistent or non-inactivating portion of sodium currents (INaP). We used dynamic clamp to test how INaR and INaP individually affect repetitive firing in adult cerebellar Purkinje neurons from male and female mice. We learned INaR does not scale repetitive firing rates due to its rapid decay at subthreshold voltages, and that subthreshold INaP is critical in regulating neuronal firing rate. Adjustments to the Nav conductance model used in these studies revealed INaP and INaR can be inversely scaled by adjusting occupancy in the slow inactivated kinetic state. Together with additional dynamic clamp experiments, these data suggest the regulation of sodium channel slow inactivation can fine-tune INaP and Purkinje neuron repetitive firing rates. Significance StatementAcross neuronal cell types, the resurgent sodium current (INaR) is often implicated in driving high rates of repetitive firing. Using dynamic clamp, we determined INaR is ineffective at driving subsequent action potentials, and that the subthreshold persistent sodium current (INaP) is the critical parameter for scaling repetitive firing rates. We propose INaR measured in native neurons may reflect a mechanism by which the magnitude of INaP is fine-tuned.

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Brown, S. P., Lawson, R. J., Moreno, J. D., Ransdell, J. L.. 2023-10-29. A reinterpretation of the relationship between persistent and resurgent sodium currents. https://doi.org/10.1101/2023.10.25.564042

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