Highly-parallel production of designer organoids by mosaic patterning of progenitors

Porter, C. M.; Qian, G. C.; Grindel, S. H.; Hughes, A. J.

doi:10.1101/2023.10.25.564017

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bioRxiv · 10.1101/2023.10.25.564017

Highly-parallel production of designer organoids by mosaic patterning of progenitors

Porter, C. M.·Qian, G. C.·Grindel, S. H.·Hughes, A. J.

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Abstract

Human organoids are a promising approach for disease modeling and regenerative medicine. However, organoid variability and limited control over morphological outcomes remain significant challenges. Here we extend a DNA velcro cell patterning approach, precisely controlling the number and ratio of human stem cell-derived progenitors contributing to nephron and mosaic nephron/ureteric bud organoids within arrays of microwells. We demonstrate long-term control over organoid size and morphology, decoupled from geometric constraints.

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Porter, C. M., Qian, G. C., Grindel, S. H., Hughes, A. J.. 2023-10-25. Highly-parallel production of designer organoids by mosaic patterning of progenitors. https://doi.org/10.1101/2023.10.25.564017

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