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bioRxiv · 10.1101/2023.10.24.563784

High-quality genome assemblies of four members of the Podospora anserina species complex

Ament-Velasquez, S. L.·Vogan, A. A.·Wallerman, O.·Hartmann, F. E.·Gautier, V.·Silar, P.·Giraud, T.·Johannesson, H.
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Abstract

The filamentous fungus Podospora anserina is a model organism used extensively in the study of molecular biology, senescence, prion biology, meiotic drive, mating-type chromosome evolution, and plant biomass degradation. It has recently been established that P. anserina is a member of a complex of seven, closely related species. In addition to P. anserina, high-quality genomic resources are available for two of these taxa. Here we provide chromosome-level annotated assemblies of the four remaining species of the complex, as well as a comprehensive dataset of annotated assemblies from a total of 28 Podospora genomes. We find that all seven species have genomes of around 35 Mbp arranged in seven chromosomes that are mostly collinear and less than 2% divergent from each other at genic regions. We further attempt to resolve their phylogenetic relationships, finding significant levels of phylogenetic conflict as expected from a rapid and recent diversification. SignificanceHere we provide a dataset of 28 annotated genomes from the P. anserina species complex, including chromosome-level assemblies of four species that lacked a reference genome. With this dataset in hand, biologists can take advantage of the molecular tools available for P. anserina to study evolutionary dynamics at the interphase between micro- and macroevolution, with particular emphasis on trait evolution, genome architecture, and speciation.

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Ament-Velasquez, S. L., Vogan, A. A., Wallerman, O., Hartmann, F. E., Gautier, V., Silar, P., Giraud, T., Johannesson, H.. 2023-10-28. High-quality genome assemblies of four members of the Podospora anserina species complex. https://doi.org/10.1101/2023.10.24.563784

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