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bioRxiv · 10.1101/2023.10.23.563614

Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaques

Kelkar, N. S.·Goldberg, B. S.·Dufloo, J.·Bruel, T.·Schwartz, O.·Hessell, A. J.·Ackerman, M. E.
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Abstract

The complement system can be viewed as a moderator of innate immunity, instructor of humoral immunity, and regulator of adaptive immunity. While sex and aging are known to affect humoral and cellular immune systems, their impact on the complement pathway in humans and rhesus macaques, a commonly used non-human primate model system, have not been well-studied. To address this knowledge gap, we analyzed serum samples from 90 humans and 75 rhesus macaques for the abundance and activity of the complement system components. While sequences of cascade proteins were highly conserved, dramatically different levels were observed between species. Whereas the low levels detected in rhesus samples raised questions about the suitability of the test, differences in levels of complement proteins were observed in male and female humans. Levels of total and antibody-dependent deposition of C1q and C3b on a glycosylated antigen differed between human and rhesus, suggesting differential recognition of glycans. Functional differences in complement-mediated lysis of antibody-sensitized cells were observed in multiple assays and showed that human females frequently exhibited higher lytic activity than human males or rhesus macaques, which typically did not exhibit such sexual dimorphism. Other differences between species and sexes were observed in more narrow contexts--for only certain antibodies, antigens, or assays. Collectively, these results expand our knowledge of sexual dimorphism in the complement system in humans, identifying differences that appear to be absent from rhesus macaques.

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Kelkar, N. S., Goldberg, B. S., Dufloo, J., Bruel, T., Schwartz, O., Hessell, A. J., Ackerman, M. E.. 2023-10-25. Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaques. https://doi.org/10.1101/2023.10.23.563614

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