Identification and full-genome assembly of novel Colorado potato beetle iflavirus and solinvi-like virus with SISPA Sequencing

Antonets, M. E.; Bodnev, S. A.; Rotskaya, U. N.; Kosman, E. S.; Tregubchak, T. V.; Bauer, T. V.; Azaev, M. S.; Kryukov, V. Y.; Antonets, D. V.

doi:10.1101/2023.10.21.563391

bioRxiv · 10.1101/2023.10.21.563391

Identification and full-genome assembly of novel Colorado potato beetle iflavirus and solinvi-like virus with SISPA Sequencing

Antonets, M. E.·Bodnev, S. A.·Rotskaya, U. N.·Kosman, E. S.·Tregubchak, T. V.·Bauer, T. V.·Azaev, M. S.·Kryukov, V. Y.·Antonets, D. V.

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Abstract

The Colorado potato beetle is one of the most devastating potato pests widespread in the world. However, its viral pathogens remain highly unexplored. Here using SISPA high-throughput sequencing of Colorado potato beetle (CPB) samples derived from prepupal larvae that died from an unknown infection, we have identified two previously unknown viruses and assembled their full-length genomic sequences. The subsequent genetic and phylogenetic analysis of the obtained sequences demonstrated that the isolated viruses, named Leptinotarsa iflavirus 1 and Leptinotarsa solinvi-like virus 1, are the novel representatives of Iflaviridae and Solinviviridae viral families, respectively. To the best of our knowledge, these are the first sequencing-confirmed insect viruses derived directly from CPB samples. And we also propose that Leptinotarsa iflavirus 1 may be associated with lethal disease in CPB.

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Antonets, M. E., Bodnev, S. A., Rotskaya, U. N., Kosman, E. S., Tregubchak, T. V., Bauer, T. V., Azaev, M. S., Kryukov, V. Y., Antonets, D. V.. 2023-10-21. Identification and full-genome assembly of novel Colorado potato beetle iflavirus and solinvi-like virus with SISPA Sequencing. https://doi.org/10.1101/2023.10.21.563391

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