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bioRxiv · 10.1101/2023.10.12.560929

Partial suppression of BCAA catabolism as a potential therapy for BCKDK deficiency

Abstract

Branched chain ketoacid dehydrogenase kinase (BCKDK) deficiency is a recently described inherited neurometabolic disorder of branched chain amino acid (BCAA) metabolism implying increased BCAA catabolism. It has been hypothesized that a severe reduction in systemic BCAA levels underlies the disease pathophysiology, and that BCAA supplementation may ameliorate disease phenotypes. To test this hypothesis, we characterized a recent mouse model of BCKDK deficiency and evaluated the efficacy of enteral BCAA supplementation in this model. Surprisingly, BCAA supplementation exacerbated neurodevelopmental deficits and did not correct biochemical abnormalities despite increasing systemic BCAA levels. These data suggest that aberrant flux through the BCAA catabolic pathway, not just BCAA insufficiency, may contribute to disease pathology. In support of this conclusion, genetic re-regulation of BCAA catabolism, through Dbt haploinsufficiency, partially rescued biochemical and behavioral phenotypes in BCKDK deficient mice. Collectively, these data raise into question assumptions widely made about the pathophysiology of BCKDK insufficiency and suggest a novel approach to develop potential therapies for this disease.

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BibTeXRIS

Ohl, L., Kuhs, A., Pluck, R., Durham, E., Noji, M., Philip, N. D., Arany, Z., Ahrens-Nicklas, R. C.. 2023-10-12. Partial suppression of BCAA catabolism as a potential therapy for BCKDK deficiency. https://doi.org/10.1101/2023.10.12.560929

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