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bioRxiv · 10.1101/2023.10.07.561329

A conserved N-terminal motif of CUL3 mediates assembly and licenses E3 ligase activation of CRL3KLHL22

Yin, Y.·Wang, W.·Liang, L.·Dai, Z.·Zuo, P.·Yu, S.·Lv, Y.·Ding, D.·Chen, H.·Jin, Y.·Mao, Y.
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Abstract

The CUL3-RING E3 ubiquitin ligases (CRL3s) play an essential role in response to extracellular nutrition and stress stimuli. The ubiquitin ligase function of CRL3s is activated through E3-E3 dimerization. However, how and why such a dimeric assembly is required for its ligase activity remains elusive. Here, we report the cryo-EM structure of the dimeric CRL3KLHL22 complex and reveal a conserved N-terminal motif in CUL3 that mediates the dimerization assembly and licenses the activation of CRL3KLHL22. Deletion of the CUL3 N-terminal motif leads to non-stochiometric assembly and impairs E3 activity of both CRL3KLHL22 and several other CRL3s. In addition, we found that the dynamics of dimeric E3-E3 superassembly generates a variable ubiquitination zone, potentially facilitating substrate recognition and ubiquitination. These findings demonstrate a CUL3 N-terminal motif-dependent E3-E3 superassembly mechanism and provide insights into the assembly and activation of CRL3s.

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Yin, Y., Wang, W., Liang, L., Dai, Z., Zuo, P., Yu, S., Lv, Y., Ding, D., Chen, H., Jin, Y., Mao, Y.. 2023-10-08. A conserved N-terminal motif of CUL3 mediates assembly and licenses E3 ligase activation of CRL3KLHL22. https://doi.org/10.1101/2023.10.07.561329

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