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bioRxiv · 10.1101/2023.09.28.560032

SETD2 maintains nuclear lamina stability to safeguard the genome

Abstract

Histone methyltransferases play essential roles in the organization and function of chromatin. They are also frequently mutated in human diseases including cancer1. One such often mutated methyltransferase, SETD2, associates co-transcriptionally with RNA polymerase II and catalyzes histone H3 lysine 36 trimethylation (H3K36me3) - a modification that contributes to gene transcription, splicing, and DNA repair2. While studies on SETD2 have largely focused on the consequences of its catalytic activity, the non-catalytic functions of SETD2 are largely unknown. Here we report a catalysis-independent function of SETD2 in maintaining nuclear lamina stability and genome integrity. We found that SETD2, via its intrinsically disordered N-terminus, associates with nuclear lamina proteins including lamin A/C, lamin B1, and emerin. Depletion of SETD2, or deletion of its N-terminus, resulted in widespread nuclear morphology abnormalities and genome stability defects that were reminiscent of a defective nuclear lamina. Mechanistically, the N-terminus of SETD2 facilitates the association of the mitotic kinase CDK1 with lamins, thereby promoting lamin phosphorylation and depolymerization required for nuclear envelope disassembly during mitosis. Taken together, our findings reveal an unanticipated link between the N-terminus of SETD2 and nuclear lamina organization that may underlie how SETD2 acts as a tumor suppressor.

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BibTeXRIS

Khan, A., Metts, J. M., Collins, L. C., Mills, C. A., Li, K., Brademeyer, A. L., Bowman, B. M., Major, M. B., Aube, J., Herring, L. E., Davis, I. J., Strahl, B. D.. 2023-09-29. SETD2 maintains nuclear lamina stability to safeguard the genome. https://doi.org/10.1101/2023.09.28.560032

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