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bioRxiv · 10.1101/2023.09.28.560001

Bacterial efflux pumps excrete SYTO-TM dyes from bacteria and lead to false-negative staining results

Minero, G. A. S.·Larsen, P. B.·Hoppe, M. E.·Meyer, R. L.
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Abstract

Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO dyes should be used with caution on live samples. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=94 SRC="FIGDIR/small/560001v1_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@1b8bab4org.highwire.dtl.DTLVardef@e9a84forg.highwire.dtl.DTLVardef@291058org.highwire.dtl.DTLVardef@1f0245d_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Minero, G. A. S., Larsen, P. B., Hoppe, M. E., Meyer, R. L.. 2023-09-28. Bacterial efflux pumps excrete SYTO-TM dyes from bacteria and lead to false-negative staining results. https://doi.org/10.1101/2023.09.28.560001

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