MultipleXed Population Selection and Enrichment single nucleus RNA sequencing (XPoSE-seq) enables sample identity retention during transcriptional profiling of rare populations

Savell, K. E.; Madangopal, R.; Saravanan, P.; Palaganas, R. G.; Woods, K. D.; Thompson, D. J.; Drake, O. R.; Brenner, M. B.; Weber, S. J.; Van Leer, E.; Choi, J. H.; Martin, T. L.; Martin, J. C.; Steinberg, M. K.; Austin, J. W.; Charendoff, C. I.; Hope, B. T.

doi:10.1101/2023.09.27.559834

bioRxiv · 10.1101/2023.09.27.559834

MultipleXed Population Selection and Enrichment single nucleus RNA sequencing (XPoSE-seq) enables sample identity retention during transcriptional profiling of rare populations

Savell, K. E.·Madangopal, R.·Saravanan, P.·Palaganas, R. G.·Woods, K. D.·Thompson, D. J.·Drake, O. R.·Brenner, M. B.·Weber, S. J.·Van Leer, E.·Choi, J. H.·Martin, T. L.·Martin, J. C.·Steinberg, M. K.·Austin, J. W.·Charendoff, C. I.·Hope, B. T.

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Abstract

Single nucleus RNA-sequencing is critical in deciphering tissue heterogeneity and identifying rare populations. However, current high throughput techniques are not optimized for rare target populations and require tradeoffs in design due to feasibility. We provide a novel snRNA pipeline, MulipleXed Population Selection and Enrichment snRNA-sequencing (XPoSE-seq), to enable targeted snRNA-seq experiments and in-depth transcriptomic characterization of rare target populations while retaining individual sample identity.

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Savell, K. E., Madangopal, R., Saravanan, P., Palaganas, R. G., Woods, K. D., Thompson, D. J., Drake, O. R., Brenner, M. B., Weber, S. J., Van Leer, E., Choi, J. H., Martin, T. L., Martin, J. C., Steinberg, M. K., Austin, J. W., Charendoff, C. I., Hope, B. T.. 2023-09-29. MultipleXed Population Selection and Enrichment single nucleus RNA sequencing (XPoSE-seq) enables sample identity retention during transcriptional profiling of rare populations. https://doi.org/10.1101/2023.09.27.559834

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