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bioRxiv · 10.1101/2023.09.18.558299

Tunable PhenoCycler Imaging of the Murine Pre-Clinical Tumour Microenvironments

Abraham, M. J.·Goncalves, C.·McCallum, P.·Gupta, V.·Preston, S. E. J.·Huang, F.·Chou, H.·Gagnon, N.·Johnson, N. A.·Miller, W. H.·Mann, K. K.·del Rincon, S. V.
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Abstract

The tumour microenvironment (TME) consists of tumour-supportive immune cells, endothelial cells, and fibroblasts. PhenoCycler, a high-plex single cell imaging platform, is used to characterize the complexity of the TME. Here, we used PhenoCycler to spatially resolve the TME of 8 routinely employed pre-clinical models of lymphoma, breast cancer, and melanoma. Our data reveal distinct TMEs in the different cancer models that were imaged, and show that cell-cell contacts differ depending on the tumour type examined. For instance, we found that the immune infiltration in a murine model of melanoma is altered in cellular organization in melanomas that become resistant to PD-1 therapy, with depletions in a number of cell-cell interactions. Furthermore, we provide detailed pipelines for the conjugation of antibodies that are optimized for PhenoCycler staining of murine FFPE tissues specifically, alongside open-source data analysis procedures. Overall, this is a valuable resource study seamlessly adaptable to any field of research involving murine models.

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Abraham, M. J., Goncalves, C., McCallum, P., Gupta, V., Preston, S. E. J., Huang, F., Chou, H., Gagnon, N., Johnson, N. A., Miller, W. H., Mann, K. K., del Rincon, S. V.. 2023-09-22. Tunable PhenoCycler Imaging of the Murine Pre-Clinical Tumour Microenvironments. https://doi.org/10.1101/2023.09.18.558299

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