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bioRxiv · 10.1101/2023.09.15.557902

MINSTED tracking of single biomolecules

Scheiderer, L.·von der Emde, H.·Hesselink, M.·Weber, M.·Hell, S. W.
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Abstract

We show that MINSTED localization, a method whereby the position of a fluorophore is identified with precisely controlled beams of a STED microscope, tracks fluorophores and hence labeled biomolecules with nanometer/millisecond spatio-temporal precision. By updating the position for each detected photon, MINSTED recognizes fluorophore steps of 16 nm within < 250 microseconds using about 13 photons. The power of MINSTED tracking is demonstrated by quantifying side-steps of the motor protein kinesin-1 walking on microtubules and switching protofilaments.

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Scheiderer, L., von der Emde, H., Hesselink, M., Weber, M., Hell, S. W.. 2023-09-15. MINSTED tracking of single biomolecules. https://doi.org/10.1101/2023.09.15.557902

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