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bioRxiv · 10.1101/2023.09.01.555969

Low-flux electron diffraction study on the intercellular lipid organization in the human lip stratum corneum

Kamimoto-Kuroki, J.·Nakazawa, H.·Ohnari, H.·Yamanoi, M.·Naru, E.·Kato, S.
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Abstract

OBJECTIVEChapped lips, characterized by drying and cracking, remain a prevalent concern. Identifying the root causes of lip chapping is crucial for developing effective treatments. We examined the lateral packing structure of intercellular lipids (ICL) in the lip stratum corneum (SC) by low-flux electron diffraction (LFED) to obtain new insights into the causes of high transepidermal water loss (TEWL) and low water retention, which may increase the vulnerability of the lip to chapping. METHODSTwenty-one healthy subjects participated in this study. After water content and TEWL measurements, a layer of corneocytes was collected from each lip vermilion surface by the grid-stripping technique. The lateral packing structure of ICL on the collected corneocytes was analyzed by LFED. RESULTSSimilar to skin SC-ICLs, we found coexistence of orthorhombic and hexagonal phases in lip SC-ICLs. We also found that electron diffraction (ED) images with no sharp peaks and a relatively small broad peak at around 2.2 nm-1 appeared frequently, unlike skin SC-ICLs. This suggests that a large fraction of corneocytes in the lip SC is surrounded by thin ICL layers in the fluid phase. Such structural features of lip SC-ICLs can explain its inferior barrier function. Furthermore, we calculated the frequency of appearance of ED images with no sharp peaks, Af, and quantitatively analyzed its correlation with water content and TEWL. The analysis showed a negative correlation between Af and water content when Af > 50%. CONCLUSIONThis is the first report on the detailed analysis of lipid organization in lip SC-ICLs. We showed that the LFED method in combination with quasi-noninvasive sample collection by the grid-stripping technique is useful for statistical study of the fine structures in lip SC. We also found that the proportion of ICLs in a fluid phase was much higher in lip SC than in skin SC, which may be related to lower water content and vulnerability of lip to chapping. Our findings provide a promising approach for obtaining clues to the structural factors regulating the water content and TEWL in lip SC, leading to more effective lip care products.

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Kamimoto-Kuroki, J., Nakazawa, H., Ohnari, H., Yamanoi, M., Naru, E., Kato, S.. 2023-09-05. Low-flux electron diffraction study on the intercellular lipid organization in the human lip stratum corneum. https://doi.org/10.1101/2023.09.01.555969

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