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bioRxiv · 10.1101/2023.08.30.555551

Discovering optimal kinetic pathways for self-assembly using automatic differentiation

Abstract

During self-assembly of macromolecules ranging from ribosomes to viral capsids, the formation of long-lived intermediates or kinetic traps can dramatically reduce yield of the functional products. Understanding biological mechanisms for avoiding traps and efficiently assembling is essential for designing synthetic assembly systems, but learning optimal solutions requires numerical searches in high-dimensional parameter spaces. Here, we exploit powerful automatic differentiation algorithms commonly employed by deep learning frameworks to optimize physical models of reversible self-assembly, discovering diverse solutions in the space of rate constants for 3-7 subunit complexes. We define two biologically-inspired protocols that prevent kinetic trapping through either internal design of subunit binding kinetics or external design of subunit titration in time. Our third protocol acts to recycle intermediates, mimicking energy-consuming enzymes. Preventative solutions via interface design are the most efficient and scale better with more subunits, but external control via titration or recycling are effective even for poorly evolved binding kinetics. Whilst all protocols can produce good solutions, diverse subunits always helps; these complexes access more efficient solutions when following external control protocols, and are simpler to design for internal control, as molecular interfaces do not need modification during assembly given sufficient variation in dimerization rates. Our results identify universal scaling in the cost of kinetic trapping, and provide multiple strategies for eliminating trapping and maximizing assembly yield across large parameter spaces. SIGNIFICANCEMacromolecular complexes are frequently composed of diverse subunits. While evolution may favor repeated subunits and symmetry, we show how diversity in subunits generates an expansive parameter space that naturally improves the expressivity of self-assembly, much like a deeper neural network. By using automatic differentiation algorithms commonly used in deep learning, we searched these parameter spaces to identify classes of kinetic protocols that mimic biological solutions for productive self-assembly. Our results reveal how high-yield complexes that easily become kinetically trapped in incomplete intermediates can instead be steered by internal design of rate constants or external and active control of subunits to efficiently assemble, exploiting nonequilibrium control of these ubiquitous dynamical systems.

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BibTeXRIS

Jhaveri, A., Loggia, S., Qian, Y., Johnson, M. E.. 2023-09-01. Discovering optimal kinetic pathways for self-assembly using automatic differentiation. https://doi.org/10.1101/2023.08.30.555551

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