Metagenomic analysis of individual mosquitos reveals the ecology of insect viruses

Pan, Y.-f.; Zhao, H.; Gou, Q.-y.; Shi, P.-b.; Tian, J.-h.; Feng, Y.; Li, K.; Yang, W.-h.; Wu, D.; Tang, G.; Zhang, B.; Ren, Z.; Peng, S.; Luo, G.-y.; Le, S.-j.; Xin, G.-y.; Wang, J.; Hou, X.; Peng, M.-w.; Kong, J.-b.; Chen, X.-x.; Yang, C.-h.; Mei, S.-q.; Liao, Y.-q.; Cheng, J.-x.; n/a, C.; Wu, Y.-h.; Wang, J.-b.; An, T.; Huang, X.; Eden, J.-S.; Li, J.; Guo, D.; Liang, G.; Jin, X.; Holmes, E. C.; Li, B.; Wang, D.; Wu, W.-c.; Shi, M.

doi:10.1101/2023.08.28.555221

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bioRxiv · 10.1101/2023.08.28.555221

Metagenomic analysis of individual mosquitos reveals the ecology of insect viruses

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Abstract

Mosquito transmitted viruses are responsible for an increasing burden of human disease. Despite this, little is known about the diversity and ecology of viruses within individual mosquito hosts. Using a meta-transcriptomic approach, we analysed the virome of 2,438 individual mosquitos (79 species), spanning [~]4000 km along latitudes and longitudes in China. From these data we identified 393 core viral species associated with mosquitos, including seven (putative) arbovirus species. We identified potential species and geographic hotspots of viral richness and arbovirus occurrence, and demonstrated that host phylogeny had a strong impact on the composition of individual mosquito viromes. Our data revealed a large number of viruses shared among mosquito species or genera, expanding our knowledge of host specificity of insect-associated viruses. We also detected multiple virus species that were widespread throughout the country, possibly facilitated by long-distance mosquito migrations. Together, our results greatly expand the known mosquito virome, linked the viral diversity at the scale of individual insects to that at a country-wide scale, and offered unique insights into the ecology of viruses of insect vectors.

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Pan, Y.-f., Zhao, H., Gou, Q.-y., Shi, P.-b., Tian, J.-h., Feng, Y., Li, K., Yang, W.-h., Wu, D., Tang, G., Zhang, B., Ren, Z., Peng, S., Luo, G.-y., Le, S.-j., Xin, G.-y., Wang, J., Hou, X., Peng, M.-w., Kong, J.-b., Chen, X.-x., Yang, C.-h., Mei, S.-q., Liao, Y.-q., Cheng, J.-x., n/a, C., Wu, Y.-h., Wang, J.-b., An, T., Huang, X., Eden, J.-S., Li, J., Guo, D., Liang, G., Jin, X., Holmes, E. C., Li, B., Wang, D., Wu, W.-c., Shi, M.. 2023-08-30. Metagenomic analysis of individual mosquitos reveals the ecology of insect viruses. https://doi.org/10.1101/2023.08.28.555221

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