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bioRxiv · 10.1101/2023.08.24.554704

Time-Resolved Cryo-EM Specimen Preparation with Single Millisecond Precision

Montano Romero, A.·Bonin, C.·Twomey, E. C.
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Abstract

Molecular structures can be determined in vitro and in situ with cryo-electron microscopy (cryo-EM). Specimen preparation is a major obstacle in cryo-EM. Typical sample preparation is orders of magnitude slower than biological processes. Time-resolved cryo-EM (TR-cryo-EM) can capture short-lived states. Here, we present Cryo-EM Sample Preparation with light-Activated Molecules (C-SPAM), an open-source, photochemistry-coupled device for TR-cryo-EM with single millisecond resolution, tunable timescales, and broad biological applications.

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Montano Romero, A., Bonin, C., Twomey, E. C.. 2023-08-24. Time-Resolved Cryo-EM Specimen Preparation with Single Millisecond Precision. https://doi.org/10.1101/2023.08.24.554704

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