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bioRxiv · 10.1101/2023.08.23.554302

Information propagation through enzyme-free catalytic templating of DNA dimerization with weak product inhibition

Cabello-Garcia, J.·Mukherjee, R.·Bae, W.·Stan, G.-B. V.·Ouldridge, T. E.
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Abstract

Information propagation by sequence-specific, template-catalyzed molecular assembly is the source of the biochemical complexity of living systems. Templating allows the production of thousands of sequence-defined proteins from only 20 distinct building blocks. By contrast, exploitation of this powerful chemical motif is rare in non-biological contexts, particularly in enzyme-free environments, where even the template-catalyzed formation of dimers is a significant challenge. The main obstacle is product inhibition: the tendency of products to bind to their templates more strongly than individual monomers, preventing the effective catalytic templating of longer polymers. Here we present a rationally designed enzyme-free system in which a DNA template catalyzes, with weak competitive product inhibition, the production of sequence-specific DNA dimers. We demonstrate the selective templating of 9 different dimers with high specificity and catalytic turnover. Most importantly, our mechanism demonstrates a rational design principle for engineering information propagation by molecular templating of longer polymers.

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Cabello-Garcia, J., Mukherjee, R., Bae, W., Stan, G.-B. V., Ouldridge, T. E.. 2023-08-24. Information propagation through enzyme-free catalytic templating of DNA dimerization with weak product inhibition. https://doi.org/10.1101/2023.08.23.554302

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