A Biotin Targeting Chimera (BioTAC) System to Map Small Molecule Interactomes in situ

Tao, A. J.; Jiang, J.; Gadbois, G. E.; Goyal, P.; Boyle, B. T.; Mumby, E. J.; Myers, S. A.; English, J. G.; Ferguson, F. M.

doi:10.1101/2023.08.21.554211

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bioRxiv · 10.1101/2023.08.21.554211

A Biotin Targeting Chimera (BioTAC) System to Map Small Molecule Interactomes in situ

Tao, A. J.·Jiang, J.·Gadbois, G. E.·Goyal, P.·Boyle, B. T.·Mumby, E. J.·Myers, S. A.·English, J. G.·Ferguson, F. M.

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Abstract

Unbiased chemical biology strategies for direct readout of protein interactome remodelling by small molecules provide advantages over target-focused approaches, including the ability to detect previously unknown targets, and the inclusion of chemical off-compete controls leading to high-confidence identifications. We describe the BioTAC system, a small-molecule guided proximity labelling platform, to rapidly identify both direct and complexed small molecule binding proteins. The BioTAC system overcomes a limitation of current approaches, and supports identification of both inhibitor bound and molecular glue bound complexes.

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Tao, A. J., Jiang, J., Gadbois, G. E., Goyal, P., Boyle, B. T., Mumby, E. J., Myers, S. A., English, J. G., Ferguson, F. M.. 2023-08-25. A Biotin Targeting Chimera (BioTAC) System to Map Small Molecule Interactomes in situ. https://doi.org/10.1101/2023.08.21.554211

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