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bioRxiv · 10.1101/2023.08.17.553737

Pneumatic Nano-Sieve for CRISPR-based Detection of Drug-resistant Bacteria

Peng, R.·Chen, X.·Xu, F.·Hailstone, R.·Men, Y.·Du, K.
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Abstract

The increasing prevalence of antibiotic-resistant bacterial infections, particularly methicillin-resistant Staphylococcus aureus (MRSA), presents a significant public health concern. Timely detection of MRSA is crucial to enable prompt medical intervention, limit its spread, and reduce antimicrobial resistance. Here, we introduce a miniaturized nano-sieve device featuring a pneumatically-regulated chamber for highly efficient MRSA purification from human plasma samples. By using packed magnetic beads as a filter and leveraging the deformability of the nano-sieve channel, we achieve an on-chip concentration factor of 15 for MRSA. We integrated this device with recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas detection system, resulting in an on-chip limit of detection (LOD) of approximately 100 CFU/mL. This developed approach provides a rapid, precise, and centrifuge-free solution suitable for point-of-care diagnostics, with the potential to significantly improve patient outcomes in resource-limited medical conditions.

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Peng, R., Chen, X., Xu, F., Hailstone, R., Men, Y., Du, K.. 2023-08-18. Pneumatic Nano-Sieve for CRISPR-based Detection of Drug-resistant Bacteria. https://doi.org/10.1101/2023.08.17.553737

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