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bioRxiv · 10.1101/2023.08.11.552859

D-serine- and p-fluorophenylalanine-resistant mutants of Physcomitrium patens are defective in amino acid uptake

Ashton, N. W.·Albone, S.·Cove, D.
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Abstract

Two amino acid analogue-resistant lines of Physcomitrium (formerly Physcomitrella) patens, DSR8 and PFR4, are resistant to a range of D-amino acids and an inhibitory concentration of L-lysine. Both are defective in the uptake of [35S]-L-methionine. Uptake by the wild-type line is pH-dependent (decreasing with raised external proton concentrations) and is depressed by dinitrophenol and by ammonium ions. We discuss the possible involvement of an active proton-general amino acid antiport pump in the plasma membrane.

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Ashton, N. W., Albone, S., Cove, D.. 2023-08-13. D-serine- and p-fluorophenylalanine-resistant mutants of Physcomitrium patens are defective in amino acid uptake. https://doi.org/10.1101/2023.08.11.552859

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