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bioRxiv · 10.1101/2023.08.09.552667

Sustained Generation of Neurons Destined for Neocortex with Oxidative Metabolic Upregulation upon Filamin Abrogation

Kopsidas, C. A.·Lowe, C. C.·McDaniel, D. P.·Zhou, X.·Feng, Y.
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Abstract

Neurons in the neocortex are generated during embryonic development. While the adult ventricular-subventricular zone (V-SVZ) contains cells with neural stem/progenitors characteristics, it remains unclear whether it has the capacity of producing neocortical neurons. Here we show that the generation of neurons exhibiting transcriptomic resemblance to neurons of the upper cortical layers continues in the V-SVZ of mouse models of a human condition known as periventricular heterotopia by abrogating filamin. We found such surplus neurogenesis was associated with V-SVZs transcriptional upregulation of oxidative phosphorylation, mitochondrial biogenesis, and increased vascularization. Our spatial transcriptomics analysis also showed that the neurogenic activation of V-SVZ was coupled with enriched expression of genes in diverse pathways for energetics, signaling, neuronal activities, and metabolic turnovers of nucleic acids and proteins in upper cortical layers. These findings support the potential of generating neocortical neurons in adulthood through enhancing brain-wide vascular circulation, aerobic ATP synthesis, and neuronal vitality.

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Kopsidas, C. A., Lowe, C. C., McDaniel, D. P., Zhou, X., Feng, Y.. 2023-08-12. Sustained Generation of Neurons Destined for Neocortex with Oxidative Metabolic Upregulation upon Filamin Abrogation. https://doi.org/10.1101/2023.08.09.552667

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