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bioRxiv · 10.1101/2023.08.09.552549

Actomyosin-II Proactively Shields Axons of the Central Nervous System from Mild Mechanical Stress

Abstract

SummaryPan et al found that actomyosin-II-driven radial contractility underpins the resilience of central axons to mild mechanical stress by suppressing the propagation and firing of injurious Ca2+ waves. Boosting actomyosin-II activity alleviates axon degeneration in mice with traumatic brain injury. Traumatic brain injury (TBI) remains a significant and unmet health challenge. However, our understanding of how neurons, particularly their fragile axons, withstand the abrupt mechanical impacts within the central nervous system remains largely unknown. Using a microfluidic device applying discrete levels of transverse forces to axons, we identified the stress levels that most axons could resist and explored their instant responses at nanoscale resolution. Mild stress induces rapid and reversible axon beading, driven by actomyosin-II-dependent radial contraction, which restricts the spreading and bursting of stress-induced Ca2+ waves. More severe stress causes irreversible focal swelling and Ca2+ overload, ultimately leading to focal axonal swelling and degeneration. Up-regulating actomyosin-II activity prevented the progression of initial injury in vivo, protecting commissural axons from degeneration in a mice TBI model. Our study established a scalable axon injury model and uncovered the critical roles of actomyosin-II in shielding neurons against detrimental mechanical stress.

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Pan, X., Lei, G., Li, J., Luan, T., Hu, Y., Chu, Y., Feng, Y., Zhan, W., Zhao, C.-x., Meunier, F. A., Liu, Y., LI, Y., Wang, T.. 2023-08-13. Actomyosin-II Proactively Shields Axons of the Central Nervous System from Mild Mechanical Stress. https://doi.org/10.1101/2023.08.09.552549

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