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bioRxiv · 10.1101/2023.07.09.548279

DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctions

Hu, Y.·Duan, Y.·Velusamy, A.·Narum, S.·Rogers, J.·Salaita, K.
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Abstract

The T cell receptor (TCR) is thought to be a mechanosensor, meaning that it transmits mechanical force to its antigen and leverages the force to amplify the specificity and magnitude of TCR signaling. The past decade has witnessed the development of molecular probes which have revealed many aspects of receptor mechanotransduction. However, most force probes are immobilized on hard substrates, thus failing to reveal mechanics in the physiological context of cell membranes. In this report, we developed DNA origami tension sensors (DOTS) which bear force sensors on a DNA origami breadboard and allow mapping of TCR mechanotransduction at dynamic intermembrane junctions. We demonstrate that TCR-antigen bonds experience 5-10 pN forces, and the mechanical events are dependent on cell state, antigen mobility, antigen potency, antigen height and F-actin activity. We tethered DOTS onto a microparticle to mechanically screen antigen in high throughput using flow cytometry. Finally, DOTS were anchored onto live B cell membranes thus producing the first quantification of TCR mechanics at authentic immune cell-cell junctions.

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Hu, Y., Duan, Y., Velusamy, A., Narum, S., Rogers, J., Salaita, K.. 2023-07-10. DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctions. https://doi.org/10.1101/2023.07.09.548279

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