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bioRxiv · 10.1101/2023.07.04.547664

The lncRNA TRG-AS1 promotes the growth of colorectal cancer cells through the regulation of P2RY10/GNA13

Luo, B.·Zhuang, L.·Sun, D.·Deng, L.·Zhang, Q.·Zhang, H.·Li, Y.
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Abstract

PurposeThe lncRNA TRG-AS1 and its co-expressed gene P2RY10 are important for colorectal cancer (CRC) occurrence and development. The purpose of our research was to explore the roles of TRG-AS1 and P2RY10 in CRC progression. MethodsThe abundance of TRG-AS1 and P2RY10 was determined in CRC cell lines. LoVo cells were transfected with si-TRG-AS1 and si-P2RY10 constructs. Subsequently, the viability, colony formation, and migration of the transfected cells were analyzed using cell counting kit-8, clonogenicity, and scratch-wound/Transwell(R) assays, respectively. Cells overexpressing GNA13 were used to further explore the relationship between TRG-AS1 and P2RY10 along with their downstream functions. Finally, nude mice were injected with different transfected cell types to observe tumor formation in vivo. ResultsTRG-AS1 and P2RY10 were significantly upregulated in HT-29 and LoVo compared to FHC cells. TRG-AS1 knockdown and P2RY10 silencing suppressed the viability, colony formation, and migration of LoVo cells. TRG-AS1 knockdown downregulated the expression of P2RY10, GNA12, and GNA13, while P2RY10 silencing downregulated the expression of TRG-AS1, GNA12, and GNA13. Additionally, GNA13 overexpression reversed the cell growth and gene expression changes in LoVo cells induced by TRG-AS1 knockdown or P2RY10 silencing. In vivo experiments revealed that CRC tumor growth was suppressed by TRG-AS1 knockdown and P2RY10 silencing. ConclusionsTRG-AS1 knockdown repressed the growth of CRC cells by regulating P2RY10 and GNA13 expression, thereby controlling CRC occurrence and development.

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Luo, B., Zhuang, L., Sun, D., Deng, L., Zhang, Q., Zhang, H., Li, Y.. 2023-07-04. The lncRNA TRG-AS1 promotes the growth of colorectal cancer cells through the regulation of P2RY10/GNA13. https://doi.org/10.1101/2023.07.04.547664

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