Insights from the Early Cretaceous: The promise of Lycoptera aDNA sequencing

Zhao, W.; Guo, Z.; Tian, Z.; Su, T.; Cao, G.; Qi, Z.; Qin, T.; Zhou, W.; Yang, J.; Chen, M.; Zhang, X.; Zhou, C.; Zhu, C.; Tang, M.; Wu, D.; Song, M.; Guo, Y.; Qiu, L.

doi:10.1101/2023.06.18.545504

bioRxiv · 10.1101/2023.06.18.545504

Insights from the Early Cretaceous: The promise of Lycoptera aDNA sequencing

Zhao, W.·Guo, Z.·Tian, Z.·Su, T.·Cao, G.·Qi, Z.·Qin, T.·Zhou, W.·Yang, J.·Chen, M.·Zhang, X.·Zhou, C.·Zhu, C.·Tang, M.·Wu, D.·Song, M.·Guo, Y.·Qiu, L.

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Abstract

Obtaining high-quality ancient DNA (aDNA) is a crucial process for advancements in molecular paleontology. However, current research is restricted to a limited range of fossil types due to DNA degradation and contamination from environmental DNA (eDNA). Utilizing the technology of nanoparticle affinity beads, we successfully extracted DNA from Lycoptera davidi fossils and obtained 1,258,901 DNA sequences. To ascertain their origins, we developed and implemented a novel protocol, the "mega screen method", designed to categorize aDNA fragments. This approach successfully identified 243 Actinopterygii species-specific fragments (SSFs), which subsequent analysis confirmed as original in situ DNA (oriDNA) sequences. Lycoptera davidi, a ray-finned fish from the Early Cretaceous Jehol Biota, dates back approximately 120 million years (Ma). Notably, the absence of deamination in certain oriDNA and paleoenvironmental DNA (paeDNA) fragments calls into question its reliability as a definitive marker for aDNA identification, a phenomenon potentially attributable to the protective effects of fossil diagenesis. Additionally, we identified a novel mechanism, "coding region sliding replication and recombination", responsible for generating new transposase. This mechanism, distinct from conventional processes such as horizontal gene transfer or vertical inheritance from parents, can facilitate a rapid genomic diversification. The transposase CDS insertion into the GAS2 gene of Anabarilius grahami offers molecular insights into the genetic interactions between ancestral carp and Lycoptera and the swift emergence of the expansion of early fish species starting from the early Cretaceous. These findings enhance our understanding of fossil DNA preservation, lay the groundwork for an ancient species DNA database, and introduce innovative tools for exploring evolutionary relationships across geological timescales.

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Zhao, W., Guo, Z., Tian, Z., Su, T., Cao, G., Qi, Z., Qin, T., Zhou, W., Yang, J., Chen, M., Zhang, X., Zhou, C., Zhu, C., Tang, M., Wu, D., Song, M., Guo, Y., Qiu, L.. 2023-06-21. Insights from the Early Cretaceous: The promise of Lycoptera aDNA sequencing. https://doi.org/10.1101/2023.06.18.545504

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