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bioRxiv · 10.1101/2023.06.18.545459

DNA conserved across animals that controls genes for embryonic development

Frith, M. C.·Ni, S.
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Abstract

DNA that controls gene expression (e.g. enhancers, promoters) has seemed almost never to be conserved between distantly-related animals, like vertebrates and arthropods. This is mysterious, because development of such animals is partly organized by homologous genes with similar complex expression patterns, termed "deep homology". Here we report twenty-five regulatory DNA segments conserved across bilaterian animals, of which seven are also conserved in cnidaria (coral and sea anemone). They control developmental genes (e.g. Nr2f, Ptch, Rfx1/3, Sall, Smad6, Sp5, Tbx2/3), including six homeobox genes: Gsx, Hmx, Meis, Msx, Six1/2, and Zfhx3/4. The segments contain perfectly or near-perfectly conserved CCAAT boxes, E-boxes, and other sequences recognized by regulatory proteins. More such DNA conservation will surely be found soon, as more genomes are published and sequence comparison is optimized. This reveals a control system for animal development conserved since the Precambrian.

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Frith, M. C., Ni, S.. 2023-06-18. DNA conserved across animals that controls genes for embryonic development. https://doi.org/10.1101/2023.06.18.545459

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