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bioRxiv · 10.1101/2023.06.12.544695

Enterovirus D68 capsid formation and stability requires acidic compartments

Abstract

Enterovirus D68 (EV-D68), a picornavirus traditionally associated with respiratory infections, has recently been linked to a polio-like paralytic condition known as acute flaccid myelitis (AFM). EV-D68 is understudied, and much of the fields understanding of this virus is based on studies of poliovirus. For poliovirus, we previously showed that low pH promotes virus capsid maturation, but here we show that, for EV-D68, inhibition of compartment acidification during a specific window of infection causes a defect in capsid formation and maintenance. These phenotypes are accompanied by radical changes in the infected cell, with viral replication organelles clustering in a tight juxtanuclear grouping. Organelle acidification is critical during a narrow window from 3-4hpi, which we have termed the "transition point," separating translation and peak RNA replication from capsid formation, maturation and egress. Our findings highlight that acidification is crucial only when vesicles convert from RNA factories to virion crucibles. ImportanceThe respiratory picornavirus enterovirus D68 is a causative agent of Acute Flaccid Myelitis, a childhood paralysis disease identified in the last decade. Poliovirus, another picornavirus associated with paralytic disease, is a fecal-oral virus which survives acidic environments when passing from host-to-host. Here we follow up on our previous work showing a requirement for acidic intracellular compartments for maturation cleavage of poliovirus particles. Enterovirus D68 requires acidic vesicles for an earlier step, assembly and maintenance of viral particles themselves. These data have strong implications for the use of acidification blocking treatments to combat enterovirus diseases.

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Galitska, G., Jassey, A., Wagner, M. A., Pollack, N., Jackson, W. T.. 2023-06-13. Enterovirus D68 capsid formation and stability requires acidic compartments. https://doi.org/10.1101/2023.06.12.544695

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