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bioRxiv · 10.1101/2023.06.08.541088

Soluble LIGHT (TNFSF14) activates endothelial cells, thereby priming the first vessel-occlusive events in acute sickle cell disease

Abstract

In sickle cell disease (SCD), the red blood cells carry a mutated form of hemoglobin (HbS) leading to altered shape and deformability. The mutation causes abnormal hemorheological properties, mechanical hemolysis, and adhesion. The chronic vascular inflammation observed in SCD and hemolysis-related endothelium activation may trigger the vaso-occlusion of blood vessels. The prothrombotic and pro-inflammatory LIGHT/TNFSF14 is a tumor necrosis factor (TNF)- superfamily cytokine implicated in various inflammatory diseases. It is expressed by various immune cells and is considered an actor in T cell-mediated immunity and immune cell recruitment. LIGHT has also been shown to activate endothelial cells (ECs) strongly. LIGHT levels are high in the plasma of SCD patients, and platelets are a major source of its circulating form. We studied a cohort of 82 homozygous adult patients with SCD (n=108 samples) to determine whether LIGHT levels were linked to the clinical state of patients included in the Basal steady state or during an Acute crisis. Soluble LIGHT levels were high in the plasma of SCD patients during acute phases of the disease, particularly during painful occlusive crises. LIGHT levels were associated with Hb levels and inflammatory markers (mainly interferon-{gamma} and tumor necrosis factor-, specifically in acute SCD patients). Our findings confirm that LIGHT is a strong activator of cultured ECs, inducing a type II inflammatory cytokine profile and the expression of adhesion molecules. Using a physiological flow adhesion test on biochips, we showed that the LIGHT-induced activation of ECs led to the adhesion of both sickle platelets (but not their AA counterparts), and in a less extend sickle RBCs to activated HUVECs, potentially constituting the first step in vaso-occlusion. Indeed, the pretreatment of HUVECs with neutralizing polyclonal Abs against LIGHT, but not the non-specific counterpart, showed a reversal of both the inflammation process activated by LIGHT treatment and platelet adhesion to endothelial cells. Soluble LIGHT appears to be a promising therapeutic target for preventing adverse occlusive events in SCD through the blockade of its receptor, to prevent the adhesion of blood cell components to the endothelium. Future studies should consider whether soluble LIGHT contributes to other clinical complications in SCD. HIGHLIGHTSO_LIIn patients with SCD, plasma LIGHT is mainly secreted during acute phases, including VOCs. C_LIO_LIBoth Hb levels and IFN{gamma} levels are correlated with plasma LIGHT with SCD patients in acute phase disease. C_LIO_LIThe LIGHT-induced activation of endothelial cells leads to the flow adherence of both sickle (SS) platelets and red blood cells. C_LIO_LIBoth endothelial priming by LIGHT and platelet adherence are abolished by anti-LIGHT polyclonal Abs. C_LI

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BibTeXRIS

Chadebech, P., Nguyen-Peyre, K.-A., Di Liberto, G., Bodivit, G., Pirenne, F., Bartolucci, P.. 2023-06-08. Soluble LIGHT (TNFSF14) activates endothelial cells, thereby priming the first vessel-occlusive events in acute sickle cell disease. https://doi.org/10.1101/2023.06.08.541088

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