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bioRxiv · 10.1101/2023.06.05.543746

Boldine alters serum lipidomic signatures after acute spinal cord transection in male mice

Graham, Z. A.·Siedlik, J. A.·Toro, C. A.·Harlow, L.·Cardozo, C. P.
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Abstract

Traumatic spinal cord injury (SCI) results in wide-ranging cellular and systemic dysfunction in the acute and chronic time frames after the injury. Chronic SCI has well-described secondary medical consequences while acute SCI has unique metabolic challenges as a result of physical trauma, in-patient recovery and other post-operative outcomes. Here, we used high resolution mass spectrometry approaches to describe the circulating lipidomic and metabolomic signatures using blood serum from mice 7 d after a complete SCI. Additionally, we probed whether the aporphine alkaloid, boldine, was able to prevent SCI-induced changes observed using these omics platforms. We found that SCI resulted in large-scale changes to the circulating lipidome but minimal changes in the metabolome, with boldine able to reverse or attenuate SCI-induced changes in the abundance of 50 lipids. Multiomic integration using xMWAS demonstrated unique network structures and community memberships across the groups.

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Graham, Z. A., Siedlik, J. A., Toro, C. A., Harlow, L., Cardozo, C. P.. 2023-06-07. Boldine alters serum lipidomic signatures after acute spinal cord transection in male mice. https://doi.org/10.1101/2023.06.05.543746

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