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bioRxiv · 10.1101/2023.06.05.543679

Aeroplane wing, a new recessive autosomal phenotypic marker in malaria vector, Anopheles stephensi Liston

Ghosh, C.·M, S.·Kumar, N.·Ramanjini, C. K.·Joshi, S. G.·S, S. K.·Subramani, S.·Swain, S.
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Abstract

A novel and distinct mutant, with an aeroplane wing (ae) phenotype, is reported for the first time in the urban malaria vector, Anopheles stephensi. The mutant mosquitoes exhibit extended wings that are easily visible to naked eyes in both sexes. This mutant was first observed in a nutritionally stressed experimental isofemale line and characterized for its genetic inheritance and other related parameters. Meticulous and strategic genetic crosses revealed that the ae gene is an autosomal, recessive, non-sex-linked and monogenic trait with full penetrance and uniform expression in the adult stage. Cytogenetic study of the ovarian polytene chromosome revealed an inversion on the 3L chromosome (3Li) in both the ae mutant and its parent line. No significant differences in wing venation and other parameters were observed in ae mutants compared to their normal parental lines. This ae mutant would be an excellent marker that can be used by researchers to study the function of related genes within the genome. Author summaryIn the present study, we have established and describe the inheritance of an unusual novel aeroplane (ae) winged mutant in Anopheles stephensi, an urban malaria vector in India. The ae mutant lines exhibit three open-wing orientations in both the sexes of adults viz. left (LW), right (RW) and double wing (DW) during its resting phase. Through various inbreeding crosses we demonstrated the mode of inheritance of the ae gene to be autosomal, recessive and monogenic in nature. The morphometric studies of eggs and wings revealed that mutant lines are on par with their parental lines. Cytogenetic study of polytene chromosome of ae mutant revealed the presence of heterogenic inversion on the 3L chromosome arm, which might help in adaptation mechanism. We strongly believe that ae phenotypic markers have great applications bridging both basic and applied genetic research such as constructing linkage maps, identifying loci of quantitative and/or qualitative traits and as guides for insect transformation studies.

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Ghosh, C., M, S., Kumar, N., Ramanjini, C. K., Joshi, S. G., S, S. K., Subramani, S., Swain, S.. 2023-06-07. Aeroplane wing, a new recessive autosomal phenotypic marker in malaria vector, Anopheles stephensi Liston. https://doi.org/10.1101/2023.06.05.543679

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