High-throughput confocal airy beam oblique light-sheet tomography of brain-wide imaging at single-cell resolution

Qi, X.; Castaneda, R. M.; Narasimhan, A.; Ding, L.; Chen, X.; Elowsky, C.; Palmer, J.; Drewes, R.; Sun, J.; Mizrachi, J.; Peng, H.; Wu, Z.; Osten, P.

doi:10.1101/2023.06.04.543586

bioRxiv · 10.1101/2023.06.04.543586

High-throughput confocal airy beam oblique light-sheet tomography of brain-wide imaging at single-cell resolution

Qi, X.·Castaneda, R. M.·Narasimhan, A.·Ding, L.·Chen, X.·Elowsky, C.·Palmer, J.·Drewes, R.·Sun, J.·Mizrachi, J.·Peng, H.·Wu, Z.·Osten, P.

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Abstract

Brain research is an area of research characterized by its cutting-edge nature, with brain mapping constituting a crucial aspect of this field. As sequencing tools have played a crucial role in gene sequencing, brain mapping largely depends on automated, high-throughput and high-resolution imaging techniques. Over the years, the demand for high-throughput imaging has scaled exponentially with the rapid development of microscopic brain mapping. In this paper, we introduce the novel concept of confocal Airy beam into oblique light-sheet tomography named CAB-OLST. We demonstrate that this technique enables the high throughput of brain-wide imaging of long-distance axon projection for the entire mouse brain at a resolution of 0.26 m x 0.26 m x 1.06 m in 58 hours. This technique represents an innovative contribution to the field of brain research by setting a new standard for high-throughput imaging techniques.

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Qi, X., Castaneda, R. M., Narasimhan, A., Ding, L., Chen, X., Elowsky, C., Palmer, J., Drewes, R., Sun, J., Mizrachi, J., Peng, H., Wu, Z., Osten, P.. 2023-06-06. High-throughput confocal airy beam oblique light-sheet tomography of brain-wide imaging at single-cell resolution. https://doi.org/10.1101/2023.06.04.543586

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