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bioRxiv · 10.1101/2023.05.24.542042

Investigating the ATP binding pocket of CX3CL1 binding protein 2 using in silico approach

Kumari, R.·Kaur, S.·Hora, R.·Mishra, P. C.
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Abstract

Plasmodium falciparum (Pf) causes the most fatal form of malaria owing to its ability to cytoadhere in the microvasculature of various organs in the body. In addition to the Pf erythrocyte membrane protein 1 (PfEMP1) family that binds diverse host receptors, CX3CL1 binding proteins 1 and 2 (CBP1 and 2) also bind the endothelial chemokine CX3CL1 to effect cytoadhesion of parasite infected erthrocytes. CBP2 is a multifaceted protein that binds nucleic acids, Pf skeleton binding protein (PfSBP1) and ATP. ATP binding to the cytoplasmic domain of CBP2 (cCBP2) induces structural changes in the protein, and hints at its role in cell signaling. In this study, we have attempted to identify the ATP binding pocket of CBP2 using an in silico approach. We have also delineated the type of interactions and amino acid residues that are likely to bind ATP. As CX3CL1 binding proteins are central to parasite biology, the obtained information is likely to form the basis for inhibitor and drug design against this molecule.

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Kumari, R., Kaur, S., Hora, R., Mishra, P. C.. 2023-05-24. Investigating the ATP binding pocket of CX3CL1 binding protein 2 using in silico approach. https://doi.org/10.1101/2023.05.24.542042

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