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bioRxiv · 10.1101/2023.05.22.541801

SLC12A9 is a lysosome-detoxifying ammonium - chloride co-transporter

Abstract

Ammonia is a ubiquitous, toxic by-product of cell metabolism. Its high membrane permeability and proton affinity causes ammonia to accumulate inside acidic lysosomes in its poorly membrane-permeant form: ammonium (NH4+). Ammonium buildup compromises lysosomal function, suggesting the existence of mechanisms that protect cells from ammonium toxicity. Here, we identified SLC12A9 as a lysosomal ammonium exporter that preserves lysosomal homeostasis. SLC12A9 knockout cells showed grossly enlarged lysosomes and elevated ammonium content. These phenotypes were reversed upon removal of the metabolic source of ammonium or dissipation of the lysosomal pH gradient. Lysosomal chloride increased in SLC12A9 knockout cells and chloride binding by SLC12A9 was required for ammonium transport. Our data indicate that SLC12A9 is a chloride-driven ammonium co-transporter that is central in an unappreciated, fundamental mechanism of lysosomal physiology that may have special relevance in tissues with elevated ammonia, such as tumors.

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BibTeXRIS

Levin-Konigsberg, R., Mitra, K., Nigam, A., Spees, K., Hivare, P., Liu, K., Kundaje, A., Krishnan, Y., Bassik, M.. 2023-05-22. SLC12A9 is a lysosome-detoxifying ammonium - chloride co-transporter. https://doi.org/10.1101/2023.05.22.541801

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