CRISPR-based engineering of RNA viruses

Nemudryi, A.; Nemudraia, A.; Nichols, J. E.; Scherffius, A. M.; Zahl, T.; Wiedenheft, B. A.

doi:10.1101/2023.05.19.541219

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bioRxiv · 10.1101/2023.05.19.541219

CRISPR-based engineering of RNA viruses

Nemudryi, A.·Nemudraia, A.·Nichols, J. E.·Scherffius, A. M.·Zahl, T.·Wiedenheft, B. A.

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Abstract

CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a new recombinant RNA technology with immediate applications for the facile engineering of RNA viruses. One-Sentence SummaryProgrammable CRISPR RNA-guided ribonucleases enable recombinant RNA technology.

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Nemudryi, A., Nemudraia, A., Nichols, J. E., Scherffius, A. M., Zahl, T., Wiedenheft, B. A.. 2023-05-20. CRISPR-based engineering of RNA viruses. https://doi.org/10.1101/2023.05.19.541219

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