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bioRxiv · 10.1101/2023.05.11.540146

Serialized On-grid Lift-In Sectioning for Tomography (SOLIST)

Erdmann, P. S.·Nguyen, T.-D. H.·Perone, G.·Vazzana, R.·Don, F. K.·Silva, M.·Sorrentino, S.·Swuec, P.·Leroux, F.·Kalebic, N.·Coscia, F.
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Abstract

Cryo-focused ion beam milling has enabled groundbreaking structural discoveries in native cells. Progress toward medically relevant applications, however, has been slow. We here present an adaptation of the cryo-lift out procedure for Serialized On-grid Lift-In Sectioning for Tomography (SOLIST), which increases throughput, reduces ice contamination, and enhances sample stability. With these improvements, new specimens, ranging from high-pressure frozen reconstituted LLPS droplets to human forebrain organoids, are accessible to cryo-electron tomography.

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Erdmann, P. S., Nguyen, T.-D. H., Perone, G., Vazzana, R., Don, F. K., Silva, M., Sorrentino, S., Swuec, P., Leroux, F., Kalebic, N., Coscia, F.. 2023-05-14. Serialized On-grid Lift-In Sectioning for Tomography (SOLIST). https://doi.org/10.1101/2023.05.11.540146

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