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bioRxiv · 10.1101/2023.05.10.539793

Laser patterning bioprinting using a light sheet-based system equipped with light sheet imaging produces long-term viable skin constructs

Hafa, L.·Breideband, L.·Posada, L. R.·Torras, N.·Martinez, E.·Stelzer, E.·Pampaloni, F.
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Abstract

This research introduces a new 3D bioprinter that incorporates live imaging of the bioprinted tissue with high resolution and high-speed capabilities. The printer employs a light sheet-based system to photocrosslink polymers into hydrogels at a printing speed of up to 0.66 mm3/s with a resolution of 15.7 {micro}m. A significant advancement of this bioprinter is its ability to track cells and bioink during crosslinking, which enables real- time evaluation of the 3D-bioprinted structures quality. Fibroblast cells were encapsulated using this method, and the viability was evaluated directly after bioprinting and seven days after encapsulation, which was found to be high (83% {+/-} 4.34%). Furthermore, a full- thickness skin construct was bioprinted and maintained in culture for 6 weeks, demonstrating the long-term viability and physiological relevance of the bioprinted tissue. The usage of solid-state laser beam scanning devices could enhance bioprintings speed and precision. This fast and accurate light-based bioprinter offers a promising platform for generating customizable 3D-printed structures with viable long-term cultures. TeaserA novel bioprinter with live imaging capability using light sheet microscopy produces viable long-term cultures with high-resolution structures. Graphical abstractGeneral workflow of bioprinting skin constructs using light sheet bioprinting. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=62 SRC="FIGDIR/small/539793v1_ufig1.gif" ALT="Figure 1"> View larger version (16K): org.highwire.dtl.DTLVardef@550133org.highwire.dtl.DTLVardef@c1cce0org.highwire.dtl.DTLVardef@171c516org.highwire.dtl.DTLVardef@424d05_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Hafa, L., Breideband, L., Posada, L. R., Torras, N., Martinez, E., Stelzer, E., Pampaloni, F.. 2023-05-12. Laser patterning bioprinting using a light sheet-based system equipped with light sheet imaging produces long-term viable skin constructs. https://doi.org/10.1101/2023.05.10.539793

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