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bioRxiv · 10.1101/2023.05.06.539686

PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 2 localizes to the Golgi

Fuchs, P.·Feixes, E.·Arruda, P.·Feitosa-Araujo, E.·Fernie, A. R.·Grefen, C.·Lichtenauer, S.·Linka, N.·de Godoy Maia, I.·Meyer, A. J.·Schilasky, S.·Sweetlove, L. J.·Wege, S.·Weber, A. P. M.·Millar, A. H.·Keech, O.·Florez-Sarasa, I.·Barreto, P.·Schwarzländer, M.
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Abstract

Mitochondria act as cellular hubs of energy transformation and metabolite conversion in most eukaryotes. Plant mitochondrial electron transport chains are particularly flexible, featuring alternative components, such as ALTERNATIVE NAD(P)H DEHYDROGENASES and ALTERNATIVE OXIDASES (AOXs), that can bypass proton translocation steps. PLANT UNCOUPLING MITOCHONDRIAL PROTEINS (named PUMPs or plant UCPs) have been identified in plants as homologues of mammalian Uncoupling Proteins (UCPs), and their biochemical and physiological roles have been investigated in the context of mitochondrial energy metabolism. To dissect UCP function in Arabidopsis, the two most conserved (UCP1 and UCP2) have been targeted in recent work by combining mutant lines to circumvent potential functional redundancy in vivo. Such approaches rely on the assumption that both proteins reside in the inner mitochondrial membrane as a prerequisite for functional redundancy. Yet, contradicting results have been reported on UCP2 localization in plants. Here we provide evidence that, conversely to UCP1, which is an abundant inner mitochondrial membrane protein, UCP2 localizes to the Golgi rather than to mitochondria. Based on multiple lines of new and prior evidence, we summarize the consensus view that we have reached and provide an example of how open, critical exchange within the research community is able to constructively address ambiguities. Our observations and considerations provide direction to the ongoing discussion about the functions of UCP proteins. They further offer new perspectives for the study of Golgi membrane transport and subcellular targeting principles of membrane proteins. Since 20 to 30 % of genes in plant genomes are predicted to encode transmembrane proteins and the function of most of those proteins has not been experimentally investigated, we highlight the importance of using independent evidence for localization as a prerequisite for understanding physiological function of membrane proteins.

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Fuchs, P., Feixes, E., Arruda, P., Feitosa-Araujo, E., Fernie, A. R., Grefen, C., Lichtenauer, S., Linka, N., de Godoy Maia, I., Meyer, A. J., Schilasky, S., Sweetlove, L. J., Wege, S., Weber, A. P. M., Millar, A. H., Keech, O., Florez-Sarasa, I., Barreto, P., Schwarzländer, M.. 2023-05-08. PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 2 localizes to the Golgi. https://doi.org/10.1101/2023.05.06.539686

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