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bioRxiv · 10.1101/2023.05.01.538513

ClearX9™: an efficient alternative to fetal bovine serum for growing animal cells in vitro

Gautam, S.·Verma, N.·Manvati, S.·Dhar, P. K.
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Abstract

Fetal Bovine Serum (FBS) is a nutrient-rich fluid that contains nutritional and macromolecular factors essential for cell growth. Every year millions of pregnant cows are slaughtered in search of FBS leading to huge environmental consequences. Here we report ClearX9 - an affordable, sustainable, ethical, and effective replacement for FBS. ClearX9 cell culture medium was used to grow HeLa (cervical cancer cells), HEK293T (embryonic kidney transformed cells) and Nthy Ori-3-1 (primary thyroid follicular transformed epithelial cells) and showed encouraging growth patterns and good cellular health. Compared with the FBS-enriched cell culture medium, ClearX9 scored positive on all the parameters suggesting ClearX9 as a credible alternative to FBS. In future, more work is required to establish the efficacy of ClearX9 in toxicology testing, bio-manufacturing, regenerative medicine, and vaccine research. HIGHLIGHTSO_LIClearX9 provides good nutritional support for the growth of animal cells C_LIO_LIClearX9 cell growth performance is comparable to the serum-enriched medium C_LIO_LIClearX9 maintains a healthy morphological profile of cells during division C_LIO_LIClearX9 generates a stress-free environment within cells C_LIO_LIClearX9 does not require animal slaughter and reduces carbon footprint C_LIO_LIClearX9 has applications in biotechnology and cell cultivated meat industry C_LI

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Gautam, S., Verma, N., Manvati, S., Dhar, P. K.. 2023-05-02. ClearX9™: an efficient alternative to fetal bovine serum for growing animal cells in vitro. https://doi.org/10.1101/2023.05.01.538513

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