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bioRxiv · 10.1101/2023.04.28.538535

High-throughput ligand profile characterization in novel biosensor cell lines expressing seven heterologous olfactory receptors for the detection of volatile plant biomarkers

Zboray, K.·Toth, A. V.·Miskolczi, T. D.·Casanova, E.·Mike, A.·Sagi, L.·Lukacs, P.
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Abstract

Agriculturally important crop plants emit a multitude of volatile organic compounds (VOCs), which are excellent indicators of their health status and/or their interaction with pathogens and pests. Here, we present the generation of a novel cellular biosensor panel for the recognition of fungal pathogen-related VOCs we had identified in the field as well as during controlled inoculations of several crop plants. The panel consists of seven stable HEK293 cell lines each expressing a functional Drosophila olfactory receptor as a biosensing element and a genetically encoded fluorescent calcium indicator protein. For high-throughput measurement of odorant binding by the cells, fluorescence response was detected in an automated 384-well microplate reader upon the injection of tester VOCs. Biosensor cell lines were characterized for their reference ligand binding in more detail, then a set of 66 VOCs was profiled on all cell lines over a concentration range of three orders of magnitude (1 M to 100 M). Forty-six VOCs (70%) evoked a response in at least one biosensor cell line and certain VOCs could activate the cell lines already from the nanomolar (ppb) concentration. Interaction profiles mapped in this study will support biosensor development for agricultural applications, but the olfactory receptor proteins may also be purified from these cell lines at sufficient yields for further processing including structure determination or coupling with artificial sensor devices.

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Zboray, K., Toth, A. V., Miskolczi, T. D., Casanova, E., Mike, A., Sagi, L., Lukacs, P.. 2023-04-28. High-throughput ligand profile characterization in novel biosensor cell lines expressing seven heterologous olfactory receptors for the detection of volatile plant biomarkers. https://doi.org/10.1101/2023.04.28.538535

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