Infant Left Amygdala Volume Is Negatively Associated with Fecal Microbiota Diversity

Aatsinki, A.-K.; Tuulari, J. J.; Munukka, E. J.; Lahti, L.; Keskitalo, A.; Kailanto, H.-M.; Nolvi, S.; Scheinin, N. M.; Saunavaara, J.; Parkkola, R.; Lewis, J. D.; Hashempour, N.; Shulist, S. J.; Karlsson, L.; Karlsson, H.

doi:10.1101/2023.04.23.537273

bioRxiv · 10.1101/2023.04.23.537273

Infant Left Amygdala Volume Is Negatively Associated with Fecal Microbiota Diversity

Aatsinki, A.-K.·Tuulari, J. J.·Munukka, E. J.·Lahti, L.·Keskitalo, A.·Kailanto, H.-M.·Nolvi, S.·Scheinin, N. M.·Saunavaara, J.·Parkkola, R.·Lewis, J. D.·Hashempour, N.·Shulist, S. J.·Karlsson, L.·Karlsson, H.

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Abstract

IntroductionRodent studies have addressed the importance of early life gut microbiota in the development of emotional and social functioning. Studies in human infants are still scarce, but associations with cognition and temperament have been reported. Neuroimaging studies have linked the amygdala with fecal microbiota diversity in infants, but crucially these studies have not covered the neonatal period, and the current study addressed this gap. MethodsThe study population included 65 infants drawn from the ongoing, general population based FinnBrain Birth Cohort Study. Brain MRI was performed around the age of one month (mean age 25 days). Fecal microbiota profiles (mean 68 days) were assessed by 16s rRNA amplicon sequencing at the age of 2.5 months. ResultsWe found a negative association between infant left amygdala volume and alpha diversity (n=52, beta =-0.0043, p=0.034, adjusted for infant sex, breastfeeding, delivery mode, age during fecal sampling, age from conception during scan, and intracranial volume, Fig.1). Amygdala volumes were not associated with beta diversity (p=0.21), nor with the abundances of individual genera when adjusted for the same covariates and multiple testing. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=112 SRC="FIGDIR/small/537273v1_fig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@bf1304org.highwire.dtl.DTLVardef@798edeorg.highwire.dtl.DTLVardef@92c040org.highwire.dtl.DTLVardef@8bc2a9_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOFigure. 1.C_FLOATNO Gut microbiota alpha diversity associates negatively with left (A), but not with right (B) amygdala volume. The grey areas depict 95% confidence intervals. C_FIG ConclusionOur results provide first evidence for associations between the brain and fecal microbiota in human neonates. Although the reported data do not allow investigation of underlying mechanisms, i.e. about the directionality of the hypothesized gut-brain connection, the reported connection encourages for future investigations of manifestations of gut-brain axis in early life.

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Aatsinki, A.-K., Tuulari, J. J., Munukka, E. J., Lahti, L., Keskitalo, A., Kailanto, H.-M., Nolvi, S., Scheinin, N. M., Saunavaara, J., Parkkola, R., Lewis, J. D., Hashempour, N., Shulist, S. J., Karlsson, L., Karlsson, H.. 2023-04-24. Infant Left Amygdala Volume Is Negatively Associated with Fecal Microbiota Diversity. https://doi.org/10.1101/2023.04.23.537273

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