Search bioRxiv⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2023.04.14.536969

Abundance of Oligoflexales bacteria is associated with algal symbiont density independent of thermal stress in Aiptasia anemones

Aguirre, E. G.·Fine, M. J.·Kenkel, C. D.
View original source ↗Export metadata ↓

Abstract

Many multicellular organisms, such as humans, plants, and invertebrates, depend on symbioses with microbes for metabolic cooperation and exchange. Reef-building corals, an ecologically important order of invertebrates, are particularly vulnerable to environmental stress in part because of their nutritive symbiosis with dinoflagellate algae, and yet also benefit from these and other microbial associations. While coral microbiomes remain difficult to study because of their complexity, the anemone Aiptasia is emerging as a simplified model. Research has demonstrated co-occurrences between microbiome composition and the abundance and type of algal symbionts in cnidarians. However, whether these patterns are the result of general stress-induced shifts or depletions of algal-associated bacteria remains unclear. Our study aimed to distinguish the effect of changes in symbiont density and thermal stress on the microbiome of symbiotic Aiptasia strain CC7 by comparing them with aposymbiotic anemones, depleted of their native symbiont, Symbiodinium linucheae. Our analysis indicated that overall, thermal stress had the greatest impact on disrupting the microbiome. We found that three bacterial classes made up most of the relative abundance (60-85 %) in all samples, but the rare microbiome fluctuated between symbiotic states and following thermal stress. We also observed that S. linucheae density correlated with abundance of Oligoflexales, suggesting these bacteria may be primary symbionts of the dinoflagellate algae. The findings of this study help expand knowledge on prospective multipartite symbioses in the cnidarian holobiont and how they respond to environmental disturbance.

Source connections

Explore related subjects

ecologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Aguirre, E. G., Fine, M. J., Kenkel, C. D.. 2023-04-17. Abundance of Oligoflexales bacteria is associated with algal symbiont density independent of thermal stress in Aiptasia anemones. https://doi.org/10.1101/2023.04.14.536969

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Operationalising the context in regenerative agriculture: decision-making and farm variability

Soil degradation is a widespread challenge that requires a broad response at the individual farm level. To ensure effectivity, the practices should be tailored to the farm context: land manager objectives and farm specific challenges. These have however been difficult to quantify. Here we demonstrate that a workable farm context can be created based on a value survey, open satellite and soil data, and published models for vegetation gross primary productivity and soil erosion. Based on the findings, despite individual differences, farmers value profitability and operational efficiency, but also biodiversity and soil health. At least the regenerative farmers surveyed also value working for the greater good more than maintaining tradition or power. In spite of wide differences in farm production orientation, we also found that each farm also had a broad variation in individual fields GPP. Most fields have a stable GPP level, which is either high or low, and that there is a 2-3-fold difference between the weakest and best producing fields indicating the potential for improving GPP by improving the growing conditions on currently weak fields. In addition, soil loss was found to be highly concentrated in critical source areas, where 10% of the field area contributed to 50% of the soil loss. Overall, open data can be linked to modelling workflows to rapidly produce a decision-making context for farmers. This facilitates benchmarking and co-learning as well as enables land managers and advisors to identify the farm context for planning effective responses to soil degradation.

ecology↗
bioRxiv2026

Fly, land, listen: Autonomous intermittent locomotion enables scalable low-noise drone ecoacoustic surveys

Ecoacoustic monitoring is enabling scientists and land managers to monitor and manage biodiversity more effectively and cost-efficiently in the face of human pressures and rapidly changing climates. Currently, most ecoacoustic surveys use manually deployed static sensors to record data, limiting the scale and reach of surveying efforts. Here we present a proof-of-concept autonomous drone platform that can use intermittent locomotion to conduct ecoacoustic surveys using an onboard sensor. Our custom prototype is able to fly, navigate, and avoid obstacles autonomously, land at a pre-determined location, record audio from an onboard microphone whilst static, before taking off and moving to the next sampling site. Autonomous navigation and operation enable greater sampling flexibility, reach, and scalability. Furthermore, by recording audio only whilst landed, noise from the drone's rotors does not mask signals or disturb animals, simplifying signal processing and downstream ecological analyses. We conducted trials in a scrubland habitat at the Knepp Estate in West Sussex, where our prototype demonstrated successful autonomous navigation and obstacle avoidance. Furthermore, we found that avian biodiversity data collected from the drone platform was comparable to that from traditional static acoustic sensor deployments, and that vocalisation patterns were not significantly impacted by the noise of the drone arriving or leaving a site. While scaled deployments of our technology would require further technical and regulatory challenges to be solved, our first demonstration of autonomous intermittent robotics-assisted ecoacoustic surveys lays the foundations for more cost-effective and far-reaching biodiversity surveys, with transformative potential for conservation, agricultural management, biosecurity, and more.

ecology↗
bioRxiv2026

Do higher-order moments improve inference of population dynamics?

Fitting mathematical models of population dynamics to microbial time-series data allows us to estimate the ecological processes and interactions taking place in the microbiome. Repeated experiments of microbial systems yield replicates which slightly differ from each other. Some of this variability arises due to the fact that births and deaths occur at random. Most prior work focuses on fitting a deterministic mathematical model to the average across replicates. We use a stochastic model to fit the variability to the observed variability across replicates. Using a simulation-driven approach, we study the conditions under which our approach allows us to infer a larger fraction of ecological parameters correctly. We observe a substantial improvement in parameter inference. Lastly, our Bayesian approach not only allows us to incorporate prior information about the system, but also provides a distribution of parameters which conveys some idea of the uncertainty of the estimates.

ecology↗