Sox11 is enriched in myogenic progenitors but dispensable for development and regeneration of skeletal muscle.

Oprescu, S. N.; Baumann, N.; Chen, X.; Sun, Q.; Zhao, Y.; Yue, F.; Wang, H.; Kuang, S.

doi:10.1101/2023.03.30.534956

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bioRxiv · 10.1101/2023.03.30.534956

Sox11 is enriched in myogenic progenitors but dispensable for development and regeneration of skeletal muscle.

Oprescu, S. N.·Baumann, N.·Chen, X.·Sun, Q.·Zhao, Y.·Yue, F.·Wang, H.·Kuang, S.

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Abstract

Transcription factors (TFs) play key roles in regulating the differentiation and function of stem cells, including muscle satellite cells (MuSCs), a resident stem cell population responsible for postnatal regeneration of the skeletal muscle. Sox11 belongs to the Sry-related HMG-box (SOX) family of TFs that play diverse roles in stem cell behavior and tissue specification. Analysis of single-cell RNA-sequencing (scRNA-seq) datasets identify a specific enrichment of Sox11 mRNA in differentiating but not quiescent MuSCs. Consistent with the scRNA-seq data, Sox11 levels increase during differentiation of murine primary myoblasts in vitro. scRNA-seq data comparing muscle regeneration in young and old mice further demonstrate that Sox11 expression is reduced in aged MuSCs. Age-related decline of Sox11 expression is associated with reduced chromatin contacts within the topologically associated domains. Unexpectedly, Myod1Cre-driven deletion of Sox11 in embryonic myoblasts has no effects on muscle development and growth, resulting in apparently healthy muscles that regenerate normally. Pax7CreER or Rosa26CreER driven (MuSC-specific or global) deletion of Sox11 in adult mice similarly has no effects on MuSC differentiation or muscle regeneration. These results identify Sox11 as a novel myogenic differentiation marker with reduced expression in quiescent and aged MuSCs, but the specific function of Sox11 in myogenesis remain to be elucidated.

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Oprescu, S. N., Baumann, N., Chen, X., Sun, Q., Zhao, Y., Yue, F., Wang, H., Kuang, S.. 2023-04-01. Sox11 is enriched in myogenic progenitors but dispensable for development and regeneration of skeletal muscle.. https://doi.org/10.1101/2023.03.30.534956

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