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bioRxiv · 10.1101/2023.03.24.534131

Hierarchical Clustering and Omics Analysis Revealed Massive Molecular Shifts in a Tree under Combined Hypoxia and Salinity

Cisse, E.-H. M.·Jiang, B. H.·Yin, L.·Miao, L. F.·Zhou, J. J.·Mekontso, F. N.·Li, D. D.·Xiang, L. S.·Yang, F.
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Abstract

Field and Greenhouse studies that attempted to describe the molecular responses of trees under waterlogging (WL) combined with salinity (ST) are quasi-inexistent. To dissect plant-specific molecular responses and patterns under SWL, we integrated transcriptional and metabolic analyses involving common differentially expressed genes (DEGs) and metabolites (DMs) patterns in Dalbergia odorifera leaflets. SWL-treated seedlings exhibited an impressively high number of DEGs and DMs compared to ST or WL. Although the main common DEGs and DMs showed a neutral pattern, gene ontology enrichment following the classification in different functional categories of SWL-transcripts displayed a predominant synergistic pattern mode. Hierarchical and silhouette analysis regrouped different morpho-physiological clusters following the treatment, SWL was mainly grouped with both single stress. SWL induced a massive shutdown of the photosynthesis apparatus through LHCBs- and PSA-related genes. Starch and plastoglobuli synthesis appeared to be drastically affected by SWL, while ABA content confirmed by ABA synthesis related-genes (ABF, ABA1 and NCED) variations showed a less-needed role. NXN (Nucleoredoxin) genes are the main factors that sustain the antioxidant system under SWL. Here we provide the first molecular responses, characterization and patterns of a tree under SWL that would significantly shed light on our understanding of the molecular mechanisms underlying combined stress.

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Cisse, E.-H. M., Jiang, B. H., Yin, L., Miao, L. F., Zhou, J. J., Mekontso, F. N., Li, D. D., Xiang, L. S., Yang, F.. 2023-03-25. Hierarchical Clustering and Omics Analysis Revealed Massive Molecular Shifts in a Tree under Combined Hypoxia and Salinity. https://doi.org/10.1101/2023.03.24.534131

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