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bioRxiv · 10.1101/2023.03.23.533992

A Single-Nucleus Atlas of Seed-to-Seed Development in Arabidopsis

Lee, T. A.·Nobori, T.·Illouz-Eliaz, N.·Xu, J.·Jow, B.·Nery, J. R.·Ecker, J. R.
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Abstract

Extensive studies of the reference plant Arabidopsis have enabled a deep understanding of tissues throughout development, yet a census of cell types and states throughout development is lacking. Here, we present a single-nucleus transcriptome atlas of seed-to-seed development employing over 800,000 nuclei, encompassing a diverse set of tissues across ten developmental stages, with spatial transcriptomic validation of the dynamic seed and silique. Cross-organ analyses revealed transcriptional conservation of cell types throughout development and heterogeneity within individual cell types influenced by organ-of-origin and developmental timing, including groups of transcription factors, suggesting gatekeeping by transcription factor activation. This atlas provides a resource for the study of cell type specification throughout the development continuum and a reference for stimulus-response and genetic perturbations at the single-cell resolution.

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Lee, T. A., Nobori, T., Illouz-Eliaz, N., Xu, J., Jow, B., Nery, J. R., Ecker, J. R.. 2023-03-24. A Single-Nucleus Atlas of Seed-to-Seed Development in Arabidopsis. https://doi.org/10.1101/2023.03.23.533992

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