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bioRxiv · 10.1101/2023.03.20.533476

Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz

Nöbauer, T.·Zhang, Y.·Kim, H.·Vaziri, A.
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Abstract

Various implementations of mesoscopes provide optical access for calcium imaging across multi-millimeter fields-of-view (FOV) in the mammalian brain. However, capturing the activity of the neuronal population within such FOVs near-simultaneously and in a volumetric fashion has remained challenging since approaches for imaging scattering brain tissues typically are based on sequential acquisition. Here, we present a modular, mesoscale light field (MesoLF) imaging hardware and software solution that allows recording from thousands of neurons within volumes of 4000 x 200 {micro}m, located at up to 400 {micro}m depth in the mouse cortex, at 18 volumes per second. Our optical design and computational approach enable up to hour-long recording of [~]10,000 neurons across multiple cortical areas in mice using workstation-grade computing resources.

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Nöbauer, T., Zhang, Y., Kim, H., Vaziri, A.. 2023-03-21. Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz. https://doi.org/10.1101/2023.03.20.533476

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