Involvement of the putative metal efflux protein YbeX in ribosomal metabolism

Sarıgül, I.; Zukova, A.; Alparslan, E.; Pihlak, M.; Remm, S.; Tenson, T.; Maiväli, U.

doi:10.1101/2023.03.20.533420

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bioRxiv · 10.1101/2023.03.20.533420

Involvement of the putative metal efflux protein YbeX in ribosomal metabolism

Sarıgül, I.·Zukova, A.·Alparslan, E.·Pihlak, M.·Remm, S.·Tenson, T.·Maiväli, U.

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Abstract

YbeX of Escherichia coli, a member of CorC protein family, is a putative Co2+/ Mg2+ efflux factor. Here, we describe several{Delta} ybeX phenotypes and report an involvement of YbeX in ribosomal metabolism. E. coli lacking ybeX has a longer lag phase on outgrowth from the stationary phase. This phenotype is heterogeneous at the individual cell level and can be rescued by supplementing the growth media with magnesium.{Delta} ybeX strain is sensitive to elevated growth temperatures and to several ribosome-targeting antibiotics, which have a common ability to induce the cold shock response in E. coli.{Delta} ybeX cells accumulate distinct 16S rRNA degradation intermediates present in both 30S particles and 70S ribosomes. We propose that a function of YbeX is maintaining the magnesium homeostasis in the cell, which is needed for proper ribosomal assembly.

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Sarıgül, I., Zukova, A., Alparslan, E., Pihlak, M., Remm, S., Tenson, T., Maiväli, U.. 2023-03-21. Involvement of the putative metal efflux protein YbeX in ribosomal metabolism. https://doi.org/10.1101/2023.03.20.533420

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